TABLE 2.
Real-time PCR detection of Eurychasma dicksonii in filamentous brown algae of various geographic originsa
| Sample source and name | Description | Microscopic diagnosis | qPCR diagnosis | EMBL accession no. (sequence length, % identityb) |
|---|---|---|---|---|
| Northern hemisphere (Dunstaffnage Bay, Oban, United Kingdom) | ||||
| Eu Oban 06-29 | Infected Ectocarpus, sampled in Oban, Scotland, May 2006 | I | + | |
| Eu Oban 06-29-7 (CCAP 4018/3) | Eurychasma isolate derived from the above; maintained in CCAP 1310/300 | I | + | FM202387 (541, 96%) |
| Ec Oban 06-29-7 (CCAP 1310/300) | Healthy Ectocarpus clonal isolate obtained from the above | NI | − | |
| Eu Oban 06-29-4 | Eurychasma isolate derived from Eu Oban 06-29; maintained in Ec Oban 06-29-5 | I | + | FM202388 (539, 99%) |
| Ec Oban 06-29-5 | Healthy Ectocarpus clonal isolate obtained from Eu Oban 06-29 | NI | − | |
| Eu Oban 06-31 | Infected Pylaiella littoralis sampled in Oban, Scotland, May 2006 | I | + | |
| Eu Oban 06-32 | Infected Pylaiella littoralis sampled in Oban, Scotland, May 2006 | I | + | |
| Eu Oban 06-33 | Infected Pylaiella littoralis sampled in Oban, Scotland, May 2006 | I | + | |
| Eu Oban 07-1 | Eurychasma isolate obtained from a field collection in June 2007; maintained in P. littoralis CCAP 1330/3 | I | + | FM202389 (530, 99%) |
| Eu Oban 07 jul | Infected Pylaiella littoralis sampled in July 2007 | I | + | |
| Eu Oban 07-2 | Eurychasma isolate obtained from the above sample, maintained in P. littoralis CCAP 1330/3 | I | + | FM202390 (1,101, 100%) |
| FS Oban 07 | Healthy Pylaiella littoralis sampled in July 2007 | NI | − | |
| Southern hemisphere | ||||
| Eu US raw | Infected Ectocarpus, sampled in Ushuaia, Argentina, January 2007 | I | + | |
| Eu US 401 | Eurychasma isolate obtained from the above | I | + | FM202392 (1,695, 98%) |
| Ec US 385 | Healthy Ectocarpus clonal isolate obtained from the above | NI | − | |
| Eu FI S63 | Mixed infected Ectocarpus and Pylaiella sampled in Stanley Harbor, Falkland Islands, United Kingdom, January 2007 | I | + | FM202391 (1,828, 99%) |
| Eu FI 64-23 | Eurychasma isolate (in Ectocarpus) derived from the above | I | + | |
| Ec FI 64-20 | Healthy Ectocarpus clonal isolate obtained from the above | NI | − | |
| Pyl FI 63-3 | Healthy Pylaiella clonal isolate obtained from the above | NI | − |
a
Abbreviations: Eu, Eurychasma; Ec, Ectocarpus; Pyl, Pylaiella; I, infected; NI, not infected; +, Eurychasma detected; −, Eurychasma not detected. Field samples are highlighted in bold; derived laboratory cultures are in lightface.