TABLE 2.
Occurrence of Wolbachia in insectarium specimens of R. pallescens, as determined by PCR
| Stage or sex of R. pallescens individual (n = 45)a | Presence of Wolbachia
|
Generation | ||
|---|---|---|---|---|
| Gonadsb | Salivary glandsc | Intestined | ||
| F | + | + | + | ND |
| F | + | + | + | ND |
| F | + | + | + | ND |
| F | + | − | + | ND |
| M | + | + | + | ND |
| F | − | − | ND | 4 |
| F | − | − | ND | 4 |
| M | + | + | + | 5 |
| M | − | − | ND | 6 |
| F | − | − | ND | 6 |
| M | − | − | ND | 6 |
| M | − | − | ND | 4 |
| M | − | − | ND | 4 |
| F | + | + | + | 5 |
| F | + | + | + | 5 |
| F | + | + | − | 5 |
| F | + | + | + | 5 |
| F | + | + | + | 4 |
| F | − | − | ND | 4 |
| F | − | − | ND | 4 |
| M | − | − | ND | 5 |
| F | − | − | ND | 5 |
| F | − | − | ND | 6 |
| M | − | − | ND | 6 |
| N | − | − | ND | 4 |
| N | − | − | ND | 4 |
| F | + | + | + | 4 |
| F | + | + | + | 4 |
| M | − | − | ND | 3 |
| F | − | − | ND | 4 |
| F | − | − | ND | 3 |
| F | + | + | + | 6 |
| F | + | + | + | 6 |
| F | + | + | ND | 3 |
| F | + | − | + | 3 |
| F | + | + | ND | ND |
| M | + | ND | ND | 3 |
| M | +e | ND | ND | 3 |
| F | − | ND | ND | 3 |
| F | − | ND | ND | 3 |
| M | − | ND | ND | 3 |
| F | +e | ND | ND | 4 |
| F | + | ND | ND | 4 |
| M | − | ND | ND | 4 |
| M | +e | ND | ND | 3 |
a
N, nymph; M, male; F, female. Two individuals were nymphs, 14 individuals were males, and 29 individuals were females.
b
Twenty-three individuals were positive, and 22 individuals were negative.
c
Sixteen individuals were positive, 20 individuals were negative, and the presence of Wolbachia was not determined (ND) for 9 individuals.
d
Fifteen individuals were positive, 1 individual was negative, and the presence of Wolbachia was not determined for 29 individuals.
e
Sample used for amplifying and sequencing fbpA.