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. 2008 Nov 14;75(2):542–546. doi: 10.1128/AEM.01816-08

FIG. 1.

FIG. 1.

Diagram of the silencing constructs used in this study. Seven silencing plasmids were constructed. The silencing approach consisted at first of individually targeting the silencing of HCf-5 and HCf-6 with pH5RNAi and pH6RNAi constructs, respectively. For the “all six hydrophobins silenced” construct, the exons of five hydrophobins were added progressively to the pH6RNAi construct. H1 to H6 represent the first exon of each hydrophobin in either the forward or reverse orientation (as shown). I5 and I6 are the first introns of HCf-5 and HCf-6, respectively. The transcription of all chimeric genes was driven by the Aspergillus nidulans Pgpd promoter and by the terminator TtrpC.