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Variation of the intracellular pH (pHin) of resting cells of E. faecium E17 when glucose was added. Cells were suspended in 50 mM potassium phosphate buffer (pH 4.5). At the time indicated by the arrow glucose was added. Control cells were preincubated for 30 min at 37°C with only phosphate buffer (line a). Cells whose FoF1ATPase activity was inhibited were preincubated for 30 min at 37°C with phosphate buffer containing 30 mM DCCD (line b).
