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Effect of tyrosine addition (at ∼100 s) to resting cells of E. faecium E17 at pH 4.5 when cells were grown in the presence of tyrosine (Cells tyr+) (line a) and in the absence of tyrosine (Cells tyr−) (×) in the growth medium. At the time indicated by the arrow, tyrosine was added to the reaction mixture to a final concentration of 0.75 μM. pHin, intracellular pH.
