E. coli strains |
|
|
CC118(RK600) |
Helper strain for triparental mating |
6 |
DH5α |
Cloning strain |
Invitrogen |
JS200(pEP Pol I) |
Mutator strain |
Addgene; 3
|
XL1-Red |
Mutator strain |
Invitrogen |
P. putida strains |
|
|
GPo12(pGEc47ΔB) |
GPo1 cured of the OCT plasmid, transformed with pGEc47ΔB; contains all genes necessary for growth on alkanes, except for the alkane hydroxylase gene |
26 |
Palk1 |
GPo12(pGEc47ΔB) adapted to growth on gaseous alkanes with pCom10_alkB |
This study |
Pcyp1 |
GPo12(pGEc47ΔB) adapted to growth on gaseous alkanes with pCom8_cyp153A6 |
This study |
Pcyp2 |
Pcyp1 further adapted to growth on gaseous alkanes with pCom8*_cyp153A6-BMO1 |
This study |
Plasmids |
|
|
pCom8 |
Broad-host-range expression vector with PalkB; GmroriT alkS
|
25 |
pCom8* |
pCom8 vector adapted for improved complementation for small-chain-length alkane-dependent host growth |
This study |
pCom10 |
Broad-host-range expression vector with PalkB; KanroriT alkS
|
25 |