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Relative growth of adapted P. putida GPo12(pGEc47ΔB) strains, expressing CYP153A6 and AlkB variants, on minimal medium plates with alkanes as the sole carbon source
| Complementing alkane monooxygenase | Days required for growth to full lawn with selected carbon source
|
||||
|---|---|---|---|---|---|
| Ethane | Propane | Butane | Pentane | Octane | |
| AlkB wild type | NGa | 5 | 5 | 3 | 2 |
| AlkB-BMO1 | NG | 5 | 3 | 6 | 7 |
| AlkB-BMO2 | NG | 5 | 2 | 4 | 8 |
| CYP153A6 wild type | NG | NG | NG | 2 | 2 |
| CYP153A6-BMO1 | NG | NG | 5 | 1.5 | 5 |
NG, no growth detected during 3-week observation.
