TABLE 3.
Absolute product concentrations after bioconversion in resting E. coli BL21(DE3) cells
| Substratea | Total hydroxylated product (μM)b
|
||||
|---|---|---|---|---|---|
| CYP153A6 | CYP153A6- BMO1 | AlkB | AlkB-BMO1 | AlkB-BMO2 | |
| Propane | 421 (59) | 244 (68) | 625 (0) | 1,048 (0) | 973 (0) |
| Butane | 356 (22) | 439 (11) | 639 (1) | 1,045 (1) | 1,586 (1) |
| Pentane | 260 (62) | 374 (49) | 606 (0) | 294 (0) | 317 (0) |
| Octane | 78 (3) | 69 (46) | 1,122 (9) | 487 (2) | 308 (1) |
a
Bioconversions with propane and butane were performed in a bioreactor for 30 min with AlkB variants and for 60 min with CYP153A6 variants. Bioconversion mixtures with pentane and octane were shaken in a glass vial for 60 min for all enzymes.
b
Values in parentheses are the percentage of 2-alkanol in the total hydroxylated product. Only 1- and 2-alkanols were formed in detectable amounts.