FIG. 3.
Effects of vancomycin on disruption of the membrane potential of Bacillus coagulans JCM 2257T caused by LnqQ (A) and nisin A (B). The solid and dotted lines show the bacteriocin-caused disruption of the membrane potential of intact and vancomycin-treated cells, respectively. The arrows indicate when bacteriocins (20 nM) were added. All experiments were performed in a buffer (250 mM sucrose, 5 mM MgSO4, 10 mM potassium phosphate; pH 7.0) containing 1 μM DiSC3(5) at 30°C. a.u., arbitrary units.