TABLE 3.

Final OD₆₀₀ values, growth rates, and l-lysine concentrations of C. glutamicum DM1729, C. glutamicum DM1933, and recombinant derivatives grown in shake flasks in minimal medium containing 4% glucose with or without different supplements after 48 h^a

C. glutamicum strain	Supplement(s)	Final OD600	μ (h−1)	Concn of l-lysine (mM)
DM1729		43 ± 4	0.25 ± 0.01	21.0 ± 0.9
DM1729 ΔC-T ilvN		44 ± 2	0.17 ± 0.01	29.7 ± 2.5
DM1729	2 mM l-valine, l-isoleucine, and l-leucine	31 ± 4	0.24 ± 0.01	24.7 ± 0.6
DM1729 ΔC-T ilvN	2 mM l-valine, l-isoleucine, and l-leucine	31 ± 2	0.24 ± 0.01	29.0 ± 1.0
DM1933		40 ± 4	0.23 ± 0.01	32.6 ± 2.3
DM1933 ΔC-T ilvN		NGb
DM1933	0.5% (wt/vol) CSL	51 ± 2	0.25 ± 0.03	37.0 ± 2.0
DM1933 ΔC-T ilvN	0.5% (wt/vol) CSL	41 ± 2	0.19 ± 0.01	50.5 ± 0.4
DM1729 ΔilvB	2 mM l-valine, l-isoleucine, and l-leucine	21 ± 3	0.20 ± 0.01	51.3 ± 1.3
DM1933	2 mM l-valine, l-isoleucine, and l-leucine	34 ± 2	0.19 ± 0.01	40.5 ± 1.3
DM1933 ΔilvB	2 mM l-valine, l-isoleucine, and l-leucine	14 ± 3	0.18 ± 0.01	62.8 ± 4.2

^aAll values are means ± standard deviations of at least three independent experiments.

^bNG, no growth.