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. 2008 Dec 1;75(2):419–427. doi: 10.1128/AEM.01844-08

TABLE 3.

Final OD600 values, growth rates, and l-lysine concentrations of C. glutamicum DM1729, C. glutamicum DM1933, and recombinant derivatives grown in shake flasks in minimal medium containing 4% glucose with or without different supplements after 48 ha

C. glutamicum strain Supplement(s) Final OD600 μ (h−1) Concn of l-lysine (mM)
DM1729 43 ± 4 0.25 ± 0.01 21.0 ± 0.9
DM1729 ΔC-T ilvN 44 ± 2 0.17 ± 0.01 29.7 ± 2.5
DM1729 2 mM l-valine, l-isoleucine, and l-leucine 31 ± 4 0.24 ± 0.01 24.7 ± 0.6
DM1729 ΔC-T ilvN 2 mM l-valine, l-isoleucine, and l-leucine 31 ± 2 0.24 ± 0.01 29.0 ± 1.0
DM1933 40 ± 4 0.23 ± 0.01 32.6 ± 2.3
DM1933 ΔC-T ilvN NGb
DM1933 0.5% (wt/vol) CSL 51 ± 2 0.25 ± 0.03 37.0 ± 2.0
DM1933 ΔC-T ilvN 0.5% (wt/vol) CSL 41 ± 2 0.19 ± 0.01 50.5 ± 0.4
DM1729 ΔilvB 2 mM l-valine, l-isoleucine, and l-leucine 21 ± 3 0.20 ± 0.01 51.3 ± 1.3
DM1933 2 mM l-valine, l-isoleucine, and l-leucine 34 ± 2 0.19 ± 0.01 40.5 ± 1.3
DM1933 ΔilvB 2 mM l-valine, l-isoleucine, and l-leucine 14 ± 3 0.18 ± 0.01 62.8 ± 4.2
a

All values are means ± standard deviations of at least three independent experiments.

b

NG, no growth.