TABLE 5.
Comparison of gene expression in C. glutamicum DM1729 ΔilvB to that in DM1729
| Locus tag | mRNA ratio | Gene | Function(s)a |
|---|---|---|---|
| Amino acid biosynthesis | |||
| cg1290 | 0.40 | metE | Homocysteine methyltransferase |
| cg1436 | 7.43 | ilvN | AHAS, small subunit |
| cg1437 | 2.68 | ilvC | Acetohydroxy acid isomeroreductase |
| cg1487 | 0.35 | leuC | 3-Isopropylmalate dehydratase, large subunit |
| cg1580 | 0.47 | argC | N-Acetyl-gamma-glutamyl-phosphate reductase |
| cg1582 | 0.38 | argB | Acetylglutamate kinase |
| cg1583 | 0.43 | argD | Acetylornithine aminotransferase |
| cg1584 | 0.32 | argF | Ornithine carbamoyltransferase |
| cg1739 | 0.39 | Glutamine amidotransferase domain | |
| Central metabolism | |||
| cg2559 | 3.25 | aceB | Malate synthase |
| cg2560 | 2.30 | aceA | Isocitrate lyase |
| cg3047 | 2.47 | ackA | Acetate kinase |
| cg3048 | 2.27 | pta | Phosphotransacetylase |
| Transcriptional | |||
| cg0156 | 0.46 | Bacterial regulatory protein, Crp family | |
| cg1486 | 0.38 | ltbR | l-Leucine and l-tryptophan biosynthesis regulator, IclR family |
| cg1585 | 0.27 | argR | l-Arginine repressor |
| cg1765 | 0.44 | sufR | Predicted transcriptional regulator for the suf operon |
| cg2320 | 2.20 | Predicted transcriptional regulator | |
| cg3303 | 2.35 | Transcriptional regulator, PadR-like family | |
| Iron metabolism | |||
| cg0771 | 0.34 | DtxR/iron-regulated lipoprotein | |
| cg0924 | 0.20 | ABC-type cobalamin/Fe3+-siderophore transport system | |
| cg0926 | 0.39 | ABC-type cobalamin/Fe3+-siderophore transport system | |
| cg0927 | 0.29 | ABC-type cobalamin/Fe3+-siderophore transport system | |
| cg0928 | 0.37 | ABC-type cobalamin/Fe3+-siderophore transport system | |
| cg1762 | 0.46 | sufC | Iron-regulated ABC transporter, ATPase subunit |
| cg1763 | 0.35 | sufD | Component of an uncharacterized iron-regulated ABC-type transporter |
| cg1764 | 0.35 | sufB | Component of an uncharacterized iron-regulated ABC-type transporter |
| cg2445 | 0.45 | Probable heme oxygenase | |
| cg3404 | 0.25 | ABC-type cobalamin/Fe3+-siderophore transport system | |
| Others | |||
| cg0277 | 0.49 | Sodium sulfate symporter transmembrane component | |
| cg0503 | 0.45 | Probable 3-dehydroquinate dehydratase | |
| cg0569 | 3.30 | Cation-transporting ATPase | |
| cg0607 | 2.95 | Hypothetical secreted protein | |
| cg0963 | 2.38 | Hypothetical protein | |
| cg1043 | 2.09 | Thiol-disulfide isomerase and thioredoxins | |
| cg1049 | 2.15 | Enoyl-coenzyme A hydratase/carnithine racemase | |
| cg1085 | 2.68 | Hypothetical protein predicted by Glimmer criteria | |
| cg1087 | 2.21 | Putative membrane protein | |
| cg1090 | 2.71 | Probable γ-glutamyltranspeptidase | |
| cg1229 | 0.45 | ABC-type cobalt transport system, permease component CbiQ | |
| cg1279 | 0.44 | Putative secreted protein | |
| cg1365 | 0.47 | atpH | H+-ATPase, δ subunit |
| cg1367 | 0.47 | atpG | ATP synthase, γ subunit |
| cg1419 | 0.49 | Putative Na+-dependent transporter | |
| cg1998 | 0.49 | cglIIR | Restriction endonuclease CGLIIR protein |
| cg2095 | 0.42 | Putative membrane protein | |
| cg2438 | 2.39 | Hypothetical protein predicted by Glimmer criteria | |
| cg2477 | 2.13 | Conserved hypothetical protein | |
| cg3256 | 0.43 | Alkanal monooxygenase, alpha chain |
a
See reference 22.