TABLE 2.
Relative quantification of gene expression in different Xylella fastidiosa mutants by quantitative real-time PCR
| Gene | Fold change in expression in X. fastidiosa mutant relative to that in wild type (mean ± SE)a
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| rpfC | rpfF | rpfF rpfC | hxfA | hxfB | fimA | pilB | fimA pilO | gumD | gumH | |
| hxfA | 2.8 ± 0.3 | 0.53 ± 0.06 | 0.8 ± 0.07 | ND | 0.82 ± 0.03 | 1.4 ± 0.1 | 1.2 ± 0.16 | 1.1 ± 0.03 | 2.55 ± 0.4 | 1.68 ± 0.1 |
| hxfB | 2.11 ± 0.5 | 0.11 ± 0.03 | 0.45 ± 0.07 | 0.9 ± 0.02 | ND | 1.3.±03 | 1.6 ± 0.2 | 1.2 ± 0.01 | 2.2 ± 0.3 | 1.4 ± 0.1 |
| fimA | 2.3 ± 0.05 | 0.3 ± 0.04 | 0.5 ± 0.1 | 1.2 ± 0.03 | 1.1 ± 0.04 | ND | 1.5 ± 0.2 | ND | 1.4 ± 0.04 | 1.3 ± 0.03 |
| pilY1 | 0.9 ± 0.03 | 1.2 ± 0.05 | 1.2 ± 0.06 | 1.1 ± 0.03 | 0.9 ± 0.04 | 1.3 ± 0.03 | ND | ND | 0.9 ± 0.03 | 1.1 ± 0.01 |
| gumJ | 3.1 ± 0.08 | 0.4 ± 0.03 | 0.6 ± 0.05 | 0.9 ± 0.01 | 0.8 ± 0.03 | 0.7 ± 0.01 | 1.2 ± 0.02 | 0.7 ± 0.03 | ND | ND |
a
Quantity of mRNA relative to that in wild-type cells. Equal amounts have a value of 1.0. Normalization was carried out using 16S rRNA as an endogenous control. ND, not determined.