FIG. 2.

Quality control of DNA preparations from two M. ulcerans strains (Agy99 and Japan ITM 8756 [Jp8756]) by agarose gel electrophoresis. Shown are ethidium bromide stainings of isolated genomic DNA, four PCR products thereof, and RNase-treated and column-purified aliquots (right panel). Primer pair MK810/MK811 was designed to yield PCR product sizes specific for either Agy99 (2 kb) or Jp8756 (0.3 kb). Primer pairs MK60/MK61, MK60/MK63, and MK60/MK35 were designed to yield identical PCR products in both strains with increasing sizes (0.17, 1.2, and 2.15 kb, respectively).