TABLE 3.
DNA yields from a variety of mycobacterial speciesa
| Species and lineage/ecotype/strain | No. of isolates | Mean DNA yield (μg) (SD) | Mean DNA quality (260/280 nm ratio) (SD) |
|---|---|---|---|
| M. ulcerans classical lineage | 34 | 19.83 (9.63) | 1.63 (0.25) |
| M. ulcerans ancestral lineage | 7 | 12.14 (7.72) | 1.74 (0.24) |
| M. marinum human isolates | 3 | 18.38 (6.58) | 1.73 (0.03) |
| M. marinum fish isolates | 4 | 19.99 (19.09) | 1.40 (0.09) |
| M. pseudoshottsii L15 | 4 | 19.36 (11.44) | 1.63 (0.14) |
| M. liflandii XT128 | 3 | 7.26 (5.86) | 1.49 (0.21) |
| M. tuberculosis Pasteur 14001.0001 | 1 | 6.22 (NA)b | 1.30 (NA) |
| M. bovis BCG ATCC 35734 | 1 | 10.78 (NA) | 1.70 (NA) |
| Overall | 57 | 14.25 (4.88) | 1.58 (0.09) |
a
The optimized protocol was applied to 20 mg(wet weight) of pellets.
b
NA, not applicable.