TABLE 4.
Detection of M. ulcerans genomic DNA after extraction from infected aquatic insectsa
| N. cimicoides sample | Total/no. (%) PCR positive
|
|
|---|---|---|
| IS2404 | IS2606 | |
| Uninfected control | 0/10 (0) | 0/10 (0) |
| Inoculated | 8/8 (100) | 8/8 (100) |
| Fed | 4/7 (57) | 4/7 (57) |
a
N. cimicoides insects were subjected to DNA extraction after experimental inoculation or feeding with M. ulcerans bacteria along with control insects (noninfected or sampled from the natural environment). M. ulcerans was identified by using two different specific loci.