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Outcross between the ΔGzMAT1 ΔGzSNF1 and ΔGzSNF1-pIGPAPA strains. The ΔGzSNF1-pIGPAPA strain served as the male for the female ΔGzMAT1 ΔGzSNF1, double deletion mutants of GzSNF1 and GzMAT1-1, respectively. Ascospores were viewed under a Bio-Rad Radiance 2000 confocal microscope. The numbers above each panel indicate the ratio of non-GFP- to GFP-producing ascospores. The scale bar represents 10 μm.
