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. 2008 Oct 24;8(1):96–103. doi: 10.1128/EC.00331-08

FIG. 6.

FIG. 6.

GalXM colocalization with WGA-binding molecules and capsular lipophilic regions. C. neoformans strain B3501 was used in all panels. (Top left panel) Fluorescence of GalXM (green) and WGA (red) shows no colocalization. The scale is 10 μm. (Top right panel) Merged bright-field and fluorescence images with GalXM (green) and lipophilic stain DiI (red) show some colocalization. The scale bar is 5 μm. (Lower left panel) Merged bright-field and fluorescence images. GalXM hyperimmune sera (green) detects capsule location overlying the beginning of budding. (Right) Voxx 3D reconstruction of the fluorescent image in the left panel of GalXM (green) and GXM (red). The scale bar is 5 μm for both lower panels.