FIG. 3.

SAR in the tail region. (A) GBAP agonist activity of the N-terminally truncated analogues (white bar) and alanine-substituted analogues (shaded bar). The activity of each 10 nM synthetic peptide was measured and normalized to that of 10 nM GBAP (black bar), as described in the legend of Fig. 2B. The averages ± standard deviations of duplicate determinations are presented. (B) GBAP antagonist activity of desQ¹N²-GBAP. E. faecalis OU510 was cultured for 5 h in THB medium containing 0 nM (negative control [black bar]), 10 nM, 100 nM, or 1,000 nM of desQ¹N²-GBAP in the presence of 10 nM of GBAP. The averages ± standard deviations of duplicate determinations are presented.