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Western blot analysis of FsrC-His6. Lactococcus lactis NZ9000 cells were cultured in the presence of 10 ng ml−1 of nisin A. After induction for 3 h, the membrane fraction was prepared as described in Materials and Methods and analyzed by Western blot analysis with anti-hexahistidine tag antibody. Lane 1, XL-Ladder marker (low range); lane 2, L. lactis NZ9000 harboring pNZ8048 (vector control); lane 3, L. lactis NZ9000 harboring pNZfsrC-his6.
