FIG. 3.

Transcriptional organization of S. enterica serovar Typhimurium MI utilization genes. Strain 14028s was grown in MM with 55.5 mM MI at 37°C, and mRNA was extracted at an OD₆₀₀ of 0.4. cDNA was amplified with reverse primers listed in Table S1 in the supplemental material. RT-PCR was performed with primer pairs specific for the indicated regions 1 to 17. All PCR products were separated by 2% agarose gel electrophoresis. As controls, PCR amplification products with genomic DNA and DNase-treated RNA samples as template are shown. Line numbers correspond to PCR product numbers depicted above the gel lanes. Arrows indicate promoters identified in this study.