TABLE 2.
Fragment cloned into pDEW201 | 14028s grown in MM + glucose
|
14028s grown in MM + MI
|
14028s ΔiolR grown in MM + glucose
|
|||||
---|---|---|---|---|---|---|---|---|
RLU/OD600 unitb | SD (%) | RLU/OD600 unit | SD (%) | Fold inductionc | RLU/OD600 unit | SD (%) | Fold induction | |
PiolR | 1.92 × 105 | 3.1 | 2.01 × 106 | 2.8 | 10.5 | 2.46 × 106 | 5.3 | 12.8 |
PiolA | 4.16 × 106 | 2.4 | 1.27 × 107 | 7.8 | 3.1 | 1.78 × 107 | 3.7 | 4.3 |
PiolE | 9.27 × 102 | 67.9 | 2.97 × 106 | 0.8 | 3,202.8 | 2.16 × 104 | 17.8 | 23.3 |
PiolG1 | 4.93 × 103 | 7.4 | 1.55 × 104 | 7.4 | 3.2 | 2.65 × 104 | 10.4 | 5.4 |
PiolC1 | 2.91 × 105 | 2.8 | 9.88 × 106 | 6.7 | 33.9 | 1.90 × 107 | 2.2 | 65.3 |
PiolD1 | 2.95 × 104 | 7.3 | 5.72 × 106 | 2.6 | 194.0 | 7.42 × 106 | 5.4 | 251.7 |
PiolG2 | 5.61 × 102 | 14.0 | 6.29 × 103 | 27.0 | 11.2 | 1.69 × 104 | 26.3 | 30.1 |
PiolI2 | 8.32 × 102 | 24.2 | 1.40 × 107 | 0.7 | 16,864.2 | 3.25 × 104 | 11.9 | 39.0 |
PiolH | 1.66 × 103 | 58.9 | 1.17 × 103 | 60.2 | 0.7 | 1.83 × 103 | 32.5 | 1.1 |
Controls | ||||||||
PargS | 2.96 × 106 | 4.0 | 1.85 × 106 | 18.3 | 0.6 | 3.41 × 106 | 10.1 | 1.2 |
Pdef | 1.13 × 106 | 2.9 | 1.11 × 106 | 5.5 | 1.0 | 1.35 × 106 | 2.8 | 1.2 |
′STM0047′ | 1.62 × 104 | 6.6 | 1.25 × 104 | 3.9 | 0.8 | 1.87 × 104 | 4.0 | 1.2 |
None | 1.58 × 103 | 9.0 | 1.36 × 103 | 11.3 | 0.9 | 1.00 × 103 | 50.4 | 0.6 |
Samples were taken from the late exponential phase.
Data are averages from three independent experiments.
Fold induction was calculated with respect to the RLU/OD600 unit values for the wild-type strain grown in glucose.