TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype/features | Reference or source |
|---|---|---|
| Yersinia enterocolitica strainsa | ||
| JB580v | ΔyenR (r− m+) | 20 |
| AJD239 | ΔyenR (r− m+) ΔytxR | 2 |
| AJD1022 | ΔyenR (r− m+) ΔaraGFB::[Φ(yopH-lacZY)] | 23 |
| AJD1685 | ΔyenR (r− m+) ΔaraGFB::[Φ(ytxA-lacZY)] | This study |
| AJD1950 | ΔyenR (r− m+) ΔaraGFB::[Φ(yscA-lacZY)] | This study |
| AJD1951 | ΔyenR (r− m+) ΔaraGFB::[Φ(lcrG-lacZY)] | This study |
| AJD2351 | ΔyenR (r− m+) ΔaraGFB::[Φ(rscB-lacZY)] | This study |
| AJD2407 | ΔyenR (r− m+) ΔaraGFB::[Φ(yopE-lacZY)] | This study |
| GY460 | ΔyenR (r− m+) ΔflhDC | 37 |
| YVM925 | ΔyenR (r− m+) Φ(ysaE-lacZYA) | 32 |
| YVM987 | ΔyenR (r− m+) Φ(sycB-lacZYA) | 32 |
| Plasmids | ||
| pBAD18-kan | KmraraBp expression vector, ColE1 ori | 16 |
| pBAD33 | CmraraBp expression vector, p15A ori | 16 |
| pVLT33 | Kmrtacp expression vector, RSF1010 ori | 14 |
| pAJD594 | tacp-ytxR in pVLT33 | This study |
| pAJD654 | araBp-ytxR in pBAD33 | 2 |
| pAJD679 | araBp-His6-ytxR in pBAD18-Km | 2 |
| pAJD905 | Cmr R6K ori mob+ (RP4) sacB1+, promoterless lacZY | 23 |
| pAJD952 | yopHp fragment in pAJD905 | 23 |
| pAJD1062 | ytxAp fragment in pAJD905 | 2 |
| pAJD1284 | yscAp fragment in pAJD905 | This study |
| pAJD1285 | lcrGp fragment in pAJD905 | This study |
| pAJD1435 | araBp-virF in pBAD33 | This study |
| pAJD1437 | rscBp fragment in pAJD905 | This study |
| pAJD1439 | tacp-rscR in pVLT33 | This study |
| pAJD1473 | yopEp fragment in pAJD905 | This study |
| pGY20 | tacp-flhDC in pVLT33 | 37 |
a
All strains listed have the virulence plasmid (pYV). In some cases pYV− derivatives were made, as described previously (13), but they are not listed in the table.