TABLE 3.
Genes regulated by CyaR
| Gene | Locus | Expt 1
|
Expt 2
|
Avg change (fold) | Gene product | ||||
|---|---|---|---|---|---|---|---|---|---|
| Level when CyaR was not expresseda | Level when CyaR was expresseda | Change (fold) | Level when CyaR was not expresseda | Level when CyaR was expresseda | Change (fold) | ||||
| uspG | b0607 | 951.8 | 647.5 | −1.47 | 1,232.6 | 448.8 | −2.75 | −2.1 | Universal stress protein |
| modC | b0765 | 297.3 | 263.8 | −1.13 | 451.2 | 111.8 | −4.04 | −2.6 | Component of a molybdenum ABC-type transporter |
| ompX | b0814 | 31,805.2 | 4,250.6 | −7.48 | 19,716 | 2,519.9 | −7.82 | −7.7 | Outer membrane protein with unknown function |
| gsiB | b0830 | 1,507.9 | 447 | −3.37 | 565.1 | 428.5 | −1.32 | −2.35 | Component of a predicted glutathione ABC-type transporter |
| nadE | b1740 | 2,776.2 | 450.9 | −6.16 | 3,628.7 | 767.8 | −4.73 | −5.4 | NAD+ synthase |
| yobD | b1820 | 388.8 | 176.5 | −2.20 | 458.8 | 229.7 | −2.00 | −2.1 | Conserved inner membrane protein with unknown function |
| yeeD | b2012 | 3,063.7 | 772.8 | −3.96 | 182.6 | 170.5 | −1.07 | −2.5 | Conserved predicted protein |
| gabD | b2661 | 531.3 | 222.6 | −2.39 | 649 | 340.6 | −1.91 | −2.1 | Succinic semialdehyde dehydrogenase |
| ygaU | b2665 | 1,307 | 864.6 | −1.51 | 1,377.9 | 455.3 | −3.03 | −2.3 | Protein with unknown function |
| yqaE | b2666 | 3,254.6 | 286.7 | −11.3 | 2,304.7 | 390.2 | −5.91 | −8.6 | Conserved predicted membrane protein |
| luxS | b2687 | 6,105.7 | 1,961.7 | −3.11 | 5,417.7 | 1,589.3 | −3.41 | −3.3 | Autoinducer-2 synthase |
| cysN | b2751 | 2,528.7 | 211.1 | −12.0 | 127.3 | 24 | −5.30 | −8.6 | Subunit of sulfate adenylyltransferase |
| cysH | b2762 | 2,125.2 | 133.2 | −16.0 | 131.4 | 27 | −4.87 | −10.4 | Phosphoadenylyl sulfate reductase |
| mscL | b3291 | 2,135.8 | 1,015.1 | −2.10 | 2,005.3 | 1,029.4 | −1.95 | −2.0 | Mechanosensitive channel that relieves pressure on the membrane |
| ibpB | b3686 | 3,952.1 | 438.7 | −9.00 | 3,387.6 | 2,764.3 | −1.23 | −5.1 | Heat shock protein |
| ibpA | b3687 | 5,866.2 | 1,651.1 | −3.55 | 7,108.9 | 5,687.2 | −1.25 | −2.4 | Heat shock protein |
| sbp | b3917 | 1,454.4 | 192.9 | −7.54 | 78 | 14.1 | −5.53 | −6.5 | Sulfate binding protein involved in sulfate import |
| ryeA | b4432 | 3,807.7 | 2,131 | −1.79 | 5,880.6 | 2,309.7 | −2.55 | −2.2 | sRNA |
| sfuC | b0066 | 97.8 | 147.8 | 1.51 | 120.3 | 339.3 | 2.82 | 2.2 | Thiamine transporter |
| ykgC | b0304 | 119.7 | 160.3 | 1.34 | 103.3 | 345.3 | 3.34 | 2.3 | Predicted oxidoreductase |
| fepA | b0584 | 467.8 | 1,247.3 | 2.67 | 1,360.1 | 1,997 | 1.47 | 2.1 | Outer membrane receptor and transport protein for enterobactin |
| fepC | b0588 | 146.1 | 491.2 | 3.36 | 543 | 647.4 | 1.19 | 2.3 | Fe3+-enterobactin ABC-type transporter |
| flu | b2000 | 279 | 11,245 | 40.3 | 277.4 | 9,614 | 34.7 | 37.0 | Outer membrane protein involved in autoaggregation |
| yeeR | b2001 | 151 | 1,897.1 | 12.6 | 130.6 | 1,461.4 | 11.2 | 12.0 | Predicted membrane protein |
| yeeS | b2002 | 9 | 598.7 | 66.5 | 45.3 | 377.7 | 8.34 | 37.4 | Predicted DNA repair protein |
a
Units are Affymetrix-reported values for gene signal.