TABLE 2.

Recombination substrates containing a mutated rrnA fragment^a

Vector	rrnA positionb	Antibiotic	Reference
pMV361ΔKan-Gm-rRNA	A2059C	Clarithromycin	40
pMV361ΔKan-Gm-rRNA	A2059G	Clarithromycin	40
pMV361ΔKan-Gm-rRNA	A2058C	Clarithromycin	40
pMV361ΔKan-Gm-rRNA	A2058G	Clarithromycin	40
pMV361ΔKan-Gm-rRNA	G1491A	Paromomycin	39
pMV361ΔKan-Gm-rRNA	G1491C	Paromomycin	39
pMV361ΔKan-Gm-rRNA	G1491T	Paromomycin	39

^aAll vectors harbor a mutated rrnA fragment, which confers antibiotic resistance (the antibiotic is shown in the middle column) upon recombination with the chromosomal wild-type rrnA gene.

^bThe rrnA position of the mutated nucleotide.