TABLE 2.
Reduced muropeptides detected in HPLC fractions
| Compound (muropeptide)a | Proposed structure | Retention time (min) | Relative peak area (%) | Neutral molecular mass (Da) of reduced compound (HPLC fractions)
|
|
|---|---|---|---|---|---|
| Calculated | Determined | ||||
| 1 | Tetrall | 21.3 | 9.3 | 941.4077 | 941.4071 |
| 2 | Tetrameso | 25.4 | 22.9 | 941.4077 | 941.4051 |
| 3 | Tetra-Xll | 43.4 | 0.5 | 1,041.4238b | 1,041.4208 |
| 4 | Tetra-Xmeso | 47.9 | 1.0 | 1,041.4238b | 1,041.4208 |
| 5 | TetraTetrall/ll | 56.5 | 2.7 | 1,864.8049 | 1,864.8039 |
| 6 | TetraTetrameso/meso | 59.8 | 6.2 | 1,864.8049 | 1,864.8028 |
| 7 | TetraTetrameso/ll | 60.6 | 3.9 | 1,864.8049 | 1,864.8038 |
| 8 | TetraTetrall/meso | 64.5 | 8.9 | 1,864.8049 | 1,864.8031 |
| 9 | TetraTetra-Y | 69.1 | 0.7 | 2,236.9694c | 2,236.9660 |
| 10 | TetraTetraTetra | 75.7 | 1.2 | 2,788.2020 | 2,788.1977 |
| 11A | TetraTetraTetra | 78.1 | 1.6 | 2,788.2020 | 2,788.1951 |
| 12 | TetraTetraTetra | 79.2 | 1.2 | 2,788.2020 | 2,788.1997 |
| 13 | TetraTetraTetra | 81.4 | 1.3 | 2,788.2020 | 2,788.1988 |
| 14 | TetraTetraAnh | 88.3 | 1.8 | 1,844.7786 | 1,844.7770 |
| 15 | TetraTetraAnh | 91.0 | 3.8 | 1,844.7786 | 1,844.7775 |
| 16 | TetraTetraAnh | 91.8 | 2.4 | 1,844.7786 | 1,844.7776 |
| 17 | TetraTetraAnh | 95.3 | 5.3 | 1,844.7786 | 1,844.7784 |
| 18A | TetraTetraTetraAnh | 99.4 | 2.1 | 2,768.1758 | 2,768.1753 |
| 19A | TetraTetraTetraAnh | 101.5 | 2.9 | 2,768.1758 | 2,768.1753 |
| 20A | TetraTetraTetraAnh | 103.0 | 2.0 | 2,768.1758 | 2,768.1743 |
| 21 | TetraTetraTetraAnh | 104.4 | 1.3 | 2,768.1758 | 2,768.1762 |
| 22 | TetraTetraTetraAnh | 105.0 | 1.4 | 2,768.1758 | 2,768.1767 |
| 23 | TetraTetraTetra(diAnh) | 128.4 | 1.0 | 2,748.1496 | 2,748.1515 |
| 11B | TetraTetra-X | 78.1 | NDd | 1,964.8209b | 1,964.8174 |
| 18B | TetraTetraTetraAnh-Y | 99.4 | NDe | 3,140.3403c | 3,140.3383 |
| 19B | TetraTetraTetraAnh-Y | 101.5 | NDf | 3,140.3403c | 3,140.3393 |
| 20B | TetraTetraTetraAnh-Y | 103.0 | NDg | 3,140.3403c | 3,140.3406 |
| 20C | TetraTetraAnh-X | 103.0 | NDg | 1,944.7947b | 1,944.7937 |
a
The numbers correspond to peak numbers in Fig. 1.
b
Molecular mass calculated by assuming that modification X is C4H4O3.
c
Molecular mass calculated by assuming that modification Y is Ala-Glu-A2pm (without H2O).
d
ND, not detected. Minor component that coelutes with 11A.
e
Minor component that coelutes with 18A.
f
Minor component that coelutes with 19A.
g
Minor component that coelutes with 20A.