FIG. 2.

Inhibition of leakage and constitutive secretion of T3SS proteins. Shigella cultures were grown in the presence of DMSO or compounds, and then culture supernatants were isolated. Samples were separated by SDS-PAGE, and the 12% gels were silver stained. The gels show “leakage” of wild-type Shigella (A), constitutive secretion of the mxiH/D73A mutant, (B) and fast constitutive secretion of the ΔipaD strain (C). The positions of the abundant T3SS proteins are indicated on the right. The SepA protein, which is secreted by an autotransporter pathway, acted as a loading control.