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. 2008 Oct 31;191(2):651–660. doi: 10.1128/JB.01083-08

FIG. 1.

FIG. 1.

In vitro promoter selectivity of holoenzymes containing hybrid σ28 proteins. (A) Construction of hybrid σ28, showing the organization of σ28Ct, σ28Ec, σ28Ec-Ct4 (σ28Ec residues 1 to 165 fused to region 4 of the σ28Ct [residues 179-253]), and σ28Ct-Ec4 (σ28Ct residues 1 to 178 fused to region 4 of σ28Ec [residues 166 to 239]). (B) Gel analysis of in vitro transcription products using the RNAP holoenzyme containing σ28Ct or hybrid σ28Ec-Ct4 protein. (C) Gel analysis of in vitro transcription products using the RNAP holoenzyme containing σ28Ec or hybrid σ28Ct-Ec4 protein. RNAP is designated by the σ28 proteins as indicated on the left. Above each lane is shown the test promoter used in the transcription assay. On the right side of each panel are shown transcripts from different test promoters. PfliC serves as an internal control in the same reaction.