TABLE 1.
DnaA binding during exponential growth and replication stress
| Locus | Mean enrichment value ± SE (fold change)a
|
|||||||
|---|---|---|---|---|---|---|---|---|
|
dnaB(Ts)
|
dnaD(Ts)
|
Wild type
|
||||||
| 32°C | 47°C | 32°C | 47°C | No treatment | HPUra treatment | 32°C | 47°C | |
| dnaA | 2.2 ± 2.3 | 42.2 ± 9.5 (18.8) | 8.8 ± 2.4 | 180 ± 130 (20.4) | 4.8 ± 0.9 | 61.4 ± 1.9 (12.7) | 6.4 ± 4.0 | 6.3 ± 3.4 (1.0) |
| sda | 1.7 ± 0.4 | 6.8 ± 1.0 (4.0) | 2.2 ± 0.5 | 15.7 ± 4.2 (7.1) | 2.0 ± 0.5 | 4.4 ± 0.1 (2.2) | 1.3 ± 0.04 | 1.5 ± 0.4 (1.2) |
| ywlC | 2.3 ± 0.3 | 9.7 ± 0.8 (4.3) | 3.4 ± 1.2 | 70.0 ± 3.1 (20.7) | 2.8 ± 0.4 | 14.4 ± 5.9 (5.1) | 1.7 ± 0.38 | 1.6 ± 0.1 (0.9) |
a
Values presented are the mean (from three independent biological replicates) enrichments ± standard errors for the indicated DNA regions (dnaA, sda, and ywlC), as measured by ChIP-PCR analysis. Data are normalized to data for the yabM locus, which does not bind DnaA specifically (Fig. 1 and data not shown). Strains used were KPL69 [dnaB(Ts)], KPL73 [dnaD(Ts)], and AG174 (wild type [with or without HPUra treatment). Cells were grown at 32°C and treated with HPUra for 60 min. Temperature shifts were to 47°C, and samples were taken after 90 min at that temperature.