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X-band EPR spectrum of dithionite-reduced rAE. Freshly reconstituted rAE (0.3 mg/ml in 100 mM Tris Cl, pH 8.0) was reduced with 2 mM sodium dithionite (final concentration). The sample was shock frozen after 2 min by immersion in liquid nitrogen. Measurement conditions: temperature, 10 K; microwave power, 3.2 μW; modulation frequency, 100 kHz; modulation amplitude, 0.8 mT; microwave frequency, 9.459 GHz.
