FIG. 3.

(A) Comparison of detection sensitivities of LAMP and PCR with Tsol-clp primer set. The standard plasmids were serially diluted from 10⁴ copies per reaction to 1 copy per reaction and amplified by LAMP (upper panel) and PCR (lower panel). The F3 and B3 primers of the Tsol-clp primer set were used in the PCR. The LAMP reactions were carried out for 60 min. Lane M, 100-bp DNA ladder marker (Promega); lanes 1 to 5 represent 10⁴, 10³, 10², 10, and 1 copy(ies)/reaction, respectively; lane 6, negative control. (B) Detection limits of target genes by LAMP with Tasi-cox1 primer set against DNA samples prepared from feces containing various numbers of T. asiatica eggs. The LAMP reactions were carried out for 90 min. Lane M, 100-bp DNA ladder marker; lane 1, negative control; lane 2, T. asiatica genomic DNA as a positive control; lanes 3 to 8 represent 5, 10, 20, 30, 40, and 50 T. asiatica eggs/g of feces, respectively.