TABLE 2.
LAMP primer sets
| Species | Primer set | Primer typea | Sequence (5′ to 3′) | Target |
|---|---|---|---|---|
| T. saginata | Tsag-cox1 | F3 | TCGGCAAATATTTAATTCCTTTG | cox1 gene |
| B3 | AAATTCTAGACGCACCCG | |||
| FIP | GCCATCGAAGGAATCAATAACCAATCTGATTTGAATTTACCCCG | |||
| BIP | GACTTTTTATCCGCCTTTGTCGTCAATGCAACGAAAACATCAAGA | |||
| T. asiatica | Tasi-cox1 | F3 | GATTTTCTTTTTTTTGATGCCCA | |
| B3 | TTGAAAATAATGACGACGACA | |||
| FIP | AGGCAAGTTTAAATCAGATAACCCATTTTGATAGGTGGTTTTGGTAA | |||
| BIP | GTGGTTGTTGATTCCTTCAATAGTTTAAGGCGGATAAAAAGTTCAC | |||
| T. solium | Tsol-cox1 | F3 | CCTATTTTAATTGGAGGTTTTGG | |
| B3 | CTACCCCACTTCCTCTTGA | |||
| FIP | CAACCATGCACTTAAAGCATTCAAATTCCATTGATAAGAGGATTATCGG | |||
| BIP | GGATGTGTTTAGGCGCTGGTACAACGAAGATGATAAAGGTG | |||
| T. saginata | Tsag-clp | F3 | GGAAGTCAAAAATCAGGTGAG | clp gene |
| T. asiatica | B3 | CGCTGATAGCTAGGCTAAC | ||
| FIP | CTCAGTCCCACCCAATCCATTTCAAATCTTCATTATGCTGCGTTAC | |||
| BIP | GCACCGTGTCATTGGTAAATTTGGTGGAGCTTACTAAGCTCTATCG | |||
| T. solium | Tsol-clp | F3 | GAGGTCAAAAATCAGGTGAGAT | |
| B3 | AATGCTCCTGACTTGGTT | |||
| FIP | AGGTGCTTTCACAATAGTCCCCTGCGTCATAGGTCTTGC | |||
| BIP | TAGTCGTTGCTTCGATAGAGCTCGCTGATATCTAGGCTAATGCTG |
a
FIP consists of the sense sequence of F2 at the 3′ end and the F1c region at the 5′ end that is complementary to the F1 region. BIP consists of a B2 region at the 3′ end that is complementary to the B2c region and the same sequence as the B1c region at the 5′ end.