TABLE 3.
RFLP analysis of the 5S-23S rRNA intergenic spacer of species from the Borrelia burgdorferi sensu lato complex
| Species | Amplicon size (bp) | In silico RFLP pattern for indicated restriction enzyme
|
|
|---|---|---|---|
| DraI | MseI | ||
| B. burgdorferi B31 | 254 | 144, 52, 29, 28 | 107, 52, 38, 29, 28 |
| B. afzelii VS461 | 246 | 174, 52, 20 | 107, 68, 51, 20 |
| B. andersonii 21133 | 266 | No site | 119, 67, 52, 28 |
| B. bissettii DN127 | 257 | 144, 53, 33, 27 | 107, 52, 38, 33, 37 |
| B. bissettii 25015 | 253 | 173, 53, 27 | 107, 52, 34, 27, 17, 12, 4 |
| B. californiensis 443 | 253 | 173, 80 | 107, 51, 37, 30, 28 |
| B. carolinensis | 253 | 173, 53, 27 | 107, 67, 52, 27 |
| B. garinii 20047 | 253 | 201, 52 | 107, 95, 51 |
| B. garinii NT29 | 253 | 144, 57, 52 | 107, 57, 51, 38 |
| B. japonica HO14 | 236 | No site | 107, 78, 51 |
| B. lusitaniae PotiB2 | 257 | 145, 83, 29 | 107, 82, 39, 29 |
| B. sinica CMN3 | 235 | 157, 49, 29 | 107, 48, 38, 29, 13 |
| B. spielmanii A14S | 225 | 158, 67 | 108, 66, 51 |
| B. tanukii Hk501 | 245 | 173, 52, 20 | 174, 51, 20 |
| B. turdi Ya501 | 248 | 144, 81, 23 | 107, 51, 38, 21, 16, 8, 7 |
| B. valaisiana VS116 | 255 | 203, 52 | 174, 51, 23, 7 |