TABLE 3.
RFLP 26S rDNA and 5.8S rDNA-ITS2 PCR products generated by BstF51, CfoI, and AluI, respectively, for Malassezia isolates included in this study
| Malassezia isolate(s) | 26SrDNA amplification product (bp) | 5.8S rDNA-ITS2 amplification product (bp) | BstF51 digest (bp) | CfoI digest (bp) | AluI digest (bp) |
|---|---|---|---|---|---|
| Strains with atypical Tween patterna | 580 | 500 | 400, 180 | 260, 130, 70 | 290, 250 |
| M. furfur CBS 1878 | 580 | 500 | 400, 180 | 260, 130, 70 | 290, 250 |
| M. furfur CBS 7019 | 580 | 500 | 400, 180 | 260, 130, 70 | 290, 250 |
| M. furfur CBS 6094 | 580 | 500 | 400, 180 | 260, 130, 70 | 290, 250 |
| M. globosa CBS 7966 | 580 | 410 | 480, 100 | 480,100 | 240, 170 |
| M. restricta CBS 7877 | 580 | 410 | 500, 70 | NRSb | NRS |
| M. pachydermatis CBS 1879 | 580 | 470 | 500, 70 | 250, 230, 100 | 360, 100 |
| M. slooffiae CBS 7956 | 580 | 430 | NRS | 250, 110, 100, 70 | 320, 110 |
| M. sympodialis CBS 7222 | 580 | 400 | 400, 180 | 390, 190 | NRS |
Isolates with the atypical pattern of Tween assimilation were 13 NL, 28 NL, 39 NL, 1828 PV, 45PV, 12 DSHIV, and 4DS.
NRS, no restriction site.