Biological relevance of intra-amniotic infection as assessed by culture-dependent and culture-independent methods. The glucose (A) and interleukin-6 (B) concentrations in AF samples positive by culture and/or by culture-independent methods are presented as percentiles with medians. The ends of the boxes define the 25th and 75th percentiles, the line inside the box defines the median, and the whiskers show the highest and the lowest values. Comparisons among groups were done by Kruskal-Wallis analysis of variance, followed by post-hoc Dunn's tests. AFC, AF culture; PCR, analysis of AF for 16S rRNA by PCR; Neg, negative result; Pos, positive result; ns, not significant.