SCCmec multiplex patterns of MR-CNS strains (31). M, molecular size markers (in kilodaltons). Lanes 1 to 4 and 21 to 24, control MRSA of type IA (locus A, upstream of the pls gene; locus G, left of the junction between IS431 and pUB110; and locus D, the dcs region), type II (loci G, D, and B, kdp operon; locus C, mecI gene), type IIIA (locus F, between Tn554 and orfX; locus E, between integrated pI258 and Tn554; and locus C) and SCCmec type IV (locus D) and internal control mecA. The sizes of amplicons, by locus, are the following: A, 495 bp; B, 284 bp; C, 209 bp; D, 342 bp; E, 243 bp; F, 414 bp; G, 381 bp; and mecA, 160 bp. Lanes 5 to 20, MRSE isolates; lanes 25 to 31 and 33, methicillin-resistant S. capitis; lane 32, methicillin-resistant S. haemolyticus; and lane 34, methicillin-resistant S. hominis.