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. 2008 Oct 29;47(1):32–37. doi: 10.1128/JCM.01085-08

FIG. 2.

FIG. 2.

Three multiplex PCRs for the SCCmec type assignment of the staphylococcal isolates from the two patients colonized with MRSA and MRSE. Lanes M1 and M2, molecular size markers. Lanes 1 to 5, control MRSA for SCCmec type IA (ccrA1, class B), SCCmec type II (ccrA2, class A), SCCmec type IIIA (ccrA3, class A), SCCmec type VI (ccrA4, class B), and SCCmec type V (ccrC and class C). (A) Multiplex PCR patterns (31). Lanes 1 to 4, controls; lanes 1a and 2a, MRSA isolates; lanes 1b, 1c, 1d, and 2b, MRSE isolates. (B) Multiplex PCR for the typing of ccr genes. Lanes 1 to 5, control MRSA isolates for ccr types. (C) Multiplex PCR for typing of mec genes (19). Lanes 1 to 3, control MRSA isolates for mec classes. Lanes 1a and 2a, MRSA (ccrC, class C); lane 1b, MRSE (ccrC, class A); lane 1c, MRSE (ccrA2, class A); lanes 1d and 2b, MRSE (ccrC, class C); lane 2a, MRSA (ccrC) and MRSE (ccrC).