TABLE 5.
Comparison of monoplex RT-PCR and multiplex rRT-PCR sensitivitiesa
| Reaction | Gene | Virus | CT limit value | Limit of TCID50 detection |
|---|---|---|---|---|
| 1 | M1 | Hong Kong/948/06 (H1N1) | 30.7 | 1 |
| 1 | M1 | Hong Kong/2268/06 (H3N2) | 30.7 | 1 |
| 1 | M1 | Shenzhen/05 (H5N1) | 29.9 | 0.1 |
| 2 | H5 HA | Shenzhen/05 (H5N1) | 25.6 | 0.01 |
| 3 | H1 HA | Hong Kong/948/06 (H1N1) | 28.5 | 0.1 |
| 3 | H3 HA | Hong Kong/2268/06 (H3N2) | 32.3 | 0.1 |
a
Assessments of sensitivity levels of monoplex RT-PCR (using the M gene) and multiplex rRT-PCR (using the HA gene) were made with IAV RNA extracted from titrated virus stocks (TCID50).