TABLE 1.
Primer pairs used in this study for MLSTa
| Primer pair name | Gene target | MLST sequence coordinatesb | MLST gene coordinatesc | Primer
|
|
|---|---|---|---|---|---|
| Orientationd | Sequence (5′-3′)a | ||||
| BCT3260 | spi | c364415-363943 | 43-184 | F | TGATCCGACCCTAGCGGATGG |
| R | TATGGTGTCGCCAGGCATTCC | ||||
| BCT3262 | ddl | c1492971-1492531 | 126-265 | F | TGCCTTCCGATATGACAGCCG |
| R | TGTAGTCATAAAAGGCAACGTCCTTGAC | ||||
| BCT3263 | ddl | c1492971-1492531 | 231-369 | F | TGAAGTTGTCAAGGACGTTGCCTT |
| R | TGCACGGAAGGCTGTTTCTGC | ||||
| BCT3257 | gdh | 1123551-1124010 | 116-256 | F | TGTGCCTTCTTTGTAGACAGCGATC |
| R | TGGAGCAAGTGGTTCTCCAAAGATAGA | ||||
| BCT3258 | gki | 600427-600909 | 160-300 | F | TTCGCAGAAGGCAAATTGCTTCA |
| R | TGTTGCTGAAGCGACTGTCTCAA | ||||
| BCT3259 | recP | c1817785-1817336 | 16-154 | F | TTAACCGCGACCGCTTTATTCTTTCA |
| R | TGACCTGGTGTTTTTGAACCCCATT | ||||
| BCT3261 | xpt | 1635367-1635850 | 201-317 | F | TGAACATCACCATGAACGAAGGCATC |
| R | TCAAAACCTTGTCCTCTGGTGAGAGG | ||||
| BCT3367 | aroE | c1232155-1231720 | 295-431 | F | TCGGATGGCGTCAGTCAGATTTC |
| R | TGCAGCTCAGAAGCATATTCTAAAGCA | ||||
a
Two primer pairs are used for the amplification of different regions of ddl.
b
All coordinates are based on the S. pneumoniae R6 complete genome sequence (GenBank no. NC 003098). MLST sequence coordinates are based on published MLST loci; “c” refers to complementary strand sequence based on the orientation of the reference genome.
c
Target regions within the MLST loci used in this study.
d
F, forward; R, reverse.