FIG. 5.
Inhibition of in vivo tumor growth after immunization. Groups of six H-2b mice were either left unimmunized or given two monthly immunizations with 100 μg HBcAg-pVAX1, HBeAg-pVAX1, or pVAX1 (empty vector) plasmid. Two weeks after the last DNA plasmid immunization, mice were inoculated with 5 × 106 RBL-5/C, RBL-5/E, or RBL-5 tumor cells given s.c. Tumor sizes were measured through the skin every 2 to 3 days after the tumor cell injection. Values are given as the mean tumor size (±SE). The area under the curve for the HBc(e)Ag-pVAX1-immunized mice challenged with RBL-5/C(E) tumor cells was statistically significantly different from the curves for the other groups (ANOVA, P < 0.01).