FIG. 8.
Inhibition of in vivo tumor growth after immunization in HBcAg-, HBeAg-, and HBc/HBeAg-dbl-Tg mice. (a) Groups of six to eight H-2b (wild-type, HBcAg-Tg, HBeAg-Tg, and HBc/eAg-Tg) mice were given two monthly immunizations with 100 μg HBcAg-pVAX1 or pVAX1 (empty vector) plasmid. Two weeks after the last DNA plasmid immunization, mice were inoculated with 5 × 106 RBL-5/C tumor cells given s.c. Tumor sizes were measured through the skin every 2 to 3 days after the tumor cell injection. Values are given as the mean tumor size (±SE). The areas under the curves were compared for statistically significantly difference by ANOVA. (b) The indicated mice were immunized as described for panel a, and spleen cells were harvested for in vitro CTL analysis as described for Fig. 4.