A. salmonicida |
|
|
JF2267 |
Virulent isolate; acrV+aopB+
|
4 |
JF2646 |
Rifr derivative of JF2267 |
6 |
JF2684 |
ΔacrV::Km; derived from JF2646 |
This study |
JF2724 |
ΔaopB::Km; derived from JF2646 |
This study |
JF2953 |
ΔaopB::Km/pMMB66HEaopB+; derived from JF2724 |
This study |
JF2956 |
ΔacrV::Km/pMMB66HEacrV+; derived from JF2684 |
This study |
E. coli |
|
|
XL1-Blue |
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB laclqZΔM15 Tn10 (Tetr)] |
5 |
BL21(DE3) |
F′ dcm ompT hsdS(rB− mB−) gal λ(DE3) |
29 |
S17-1 |
thi pro hsdR hsdM+ recA [RP4 2-Tc::Mu-Km::Tn7 (Tpr Smr) Tra+] |
28 |
Plasmids |
|
|
pBSK |
Bluescript II SK−; cloning and sequencing |
Stratagene |
pGEM-T |
Cloning vector |
Promega |
pETHIS-1 |
Expression vector |
Novagen |
pSSVI186 |
Kanamycin resistance determinant on 1.3-kb PstI fragment from Tn903
|
33 |
pSUP202sac |
Mobilizable suicide vector; confers SucS (Mob+sacRB+ Tetr) |
32 |
pMMB66HE |
Broad-host-range expression vector (Ampr) |
10 |