TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Properties | Source or reference |
|---|---|---|
| A. salmonicida | ||
| JF2267 | Virulent isolate; acrV+aopB+ | 4 |
| JF2646 | Rifr derivative of JF2267 | 6 |
| JF2684 | ΔacrV::Km; derived from JF2646 | This study |
| JF2724 | ΔaopB::Km; derived from JF2646 | This study |
| JF2953 | ΔaopB::Km/pMMB66HEaopB+; derived from JF2724 | This study |
| JF2956 | ΔacrV::Km/pMMB66HEacrV+; derived from JF2684 | This study |
| E. coli | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB laclqZΔM15 Tn10 (Tetr)] | 5 |
| BL21(DE3) | F′ dcm ompT hsdS(rB− mB−) gal λ(DE3) | 29 |
| S17-1 | thi pro hsdR hsdM+ recA [RP4 2-Tc::Mu-Km::Tn7 (Tpr Smr) Tra+] | 28 |
| Plasmids | ||
| pBSK | Bluescript II SK−; cloning and sequencing | Stratagene |
| pGEM-T | Cloning vector | Promega |
| pETHIS-1 | Expression vector | Novagen |
| pSSVI186 | Kanamycin resistance determinant on 1.3-kb PstI fragment from Tn903 | 33 |
| pSUP202sac | Mobilizable suicide vector; confers SucS (Mob+sacRB+ Tetr) | 32 |
| pMMB66HE | Broad-host-range expression vector (Ampr) | 10 |