FIG. 3.

The pre-P region is translated in the S10⁻ background. An HA epitope tag was inserted 21 nt downstream of C13 in frame with P, the expression vectors were transfected into LMH cells, and P expression was measured by Western analysis of cell lysates. (A) Diagram of mutation sites on the DHBV3 pgRNA. (B) Detection of pre-P by anti-HA antibody 3F10. (C) Detection of P and pre-P by anti-P antibody MAb 11. PBS, pBluescript (empty vector control); WT, wild type (DHBV C⁻); HA, HA epitope tag. The codons from which the various P isoforms initiate are indicated.