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Binding affinities of anti-B5 MAbs. Titration of anti-B5 MAbs using IgG1-specific (A) and IgG2a-specific (B) ELISAs. (C) Mean fluorescence intensity (MFI) of cell-based ELISA quantitating surface-bound anti-B5 MAbs to native B5 on VACV-infected cells. Serial dilution of each anti-B5 MAb was performed. Data are representative of three independent experiments. OD, optical density.
