Skip to main content
. 2008 Nov 19;83(3):1492–1500. doi: 10.1128/JVI.00671-08

FIG. 4.

FIG. 4.

TLR2 signaling influences early innate cellular response against RSV in vivo. Wild-type, TLR2 KO, and MyD88 KO mice were infected by i.n. inoculation with RSV strain A2 (2.4 × 106 PFU/mouse). Uninfected mice (n = 3) were used to control for lung cytokine production and BAL infiltrate for each group. At 24, 48, and 96 h after infection, BAL and lung tissue were harvested from mice. (a) Lung tissue was homogenized and tested for CCL2 production by ELISA (wild-type and TLR2 KO mice, n = 7; MyD88 KO mice, n = 6). Data are represented as picograms/gram of lung tissue. Solid lines represent the means for each group. Error bars are ± SEM. BAL from each group (n = 3 per group) was pooled, and neutrophils (b) and activated DCs (c) were enumerated by flow cytometry. Neutrophil (7.4+ F4/80−) and DC (CD11b+ CD11c+ CD86+) populations are indicated as the percent positive cells of the total number of gated cells.