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Time course of LAT-encoded miRNA expression in LAT mutant virus- and wild-type virus-infected cells. Vero cells were infected in triplicate with HSV-2 strain 333 wild-type virus or a mutant in which the LAT promoter (NotI-SalI sequences) was deleted. Total RNA was prepared at 0, 3, 6, 9, 14, and 18 hpi. Fifty nanograms of total RNA was used in specific real-time PCR systems to quantify miR-I, miR-II, and miR-III at each time point for each virus.
