TABLE 1.
New HSV-2 LAT-encoded miRNAs identified by 454 HTP sequencing
| Viral miRNAa | Frequencyb for:
|
||
|---|---|---|---|
| pSSK libraryc | pCMV-SSK libraryc | Total | |
| miR-Id | 34 | 44 | 78 |
| miR-I-5p | 0 | 2 | 2 |
| miR-II-5p | 3 | 3 | 6 |
| miR-II-3p | 0 | 4 | 4 |
| miR-III | 3 | 5 | 8 |
a
See Fig. 1 for the sequences of these miRNAs.
b
Total number of times the miRNA-like sequences appeared in the clones sequenced.
c
Small RNA libraries from pSSK- or pCMV-SSK-transfected HEK 293 cells were constructed as described previously (40). The qualified 454 sequencing reads were approximately 16,000 for the pSSK library and 19,000 for the pCMV-SSK library, respectively.
d
miR-I has been identified and described before (40).