FIG. 10.

HSV-1 transcripts were reduced in abundance in infections with ICP27 mutants dLeu, d4-5, and n406. HeLa cells were infected with wild-type (WT) KOS or with mutant dLeu, d4-5, or n406 as indicated for 8 h. RNA was harvested from total cell lysates, and poly(A)⁺ RNA was selected. Hybridizations were performed by using HSV-1-specific microarray chips, and data were analyzed as described in the legend to Fig. 7. Experiments were performed in triplicate; error bars show standard deviations. Immediate early transcripts are shown as red bars, early transcripts as green bars, and late transcripts as blue bars.