FIG. 4.

Viral gC RNA was retained in the nucleus in cells infected with the RGG box deletion mutant d4-5. HeLa cells were either mock infected or infected with wild-type (WT) HSV-1 or mutant d4-5 for 8 h. Cells were hybridized with a biotin-labeled, locked-nucleic acid probe specific to the gC transcript. The probe was visualized with a streptavidin-Texas red-conjugated antibody. Staining with antibody to Aly/REF (to mark nuclei) or ICP27 followed. Right panels: mock-infected and wild-type HSV-1-infected cells were either treated or were not treated with RNase as indicated and then hybridized with an oligo(dT) or gC probe.