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. 2008 Nov 19;83(3):1240–1259. doi: 10.1128/JVI.01743-08

FIG. 4.

FIG. 4.

Neutralization of HIV-2KR.X7/HIV-1 V3 chimeras by HIV-1 MAbs. HIV-2KR.X7/HIV-1 V3 chimeras and control viruses were tested for neutralization susceptibility to HIV-1 MAbs targeting the CD4bs (b12), MPER (2F5, 4E10), and CD4i (17b, 19e, 21c, E51, 4.12D, ED47, ED49) epitopes and V3 (447-52D and F425 B4e8). Fivefold serial MAb dilutions were prepared at a starting concentration of 20 μg/ml, mixed with an equal volume of infectious virus stock to give the final concentrations shown, and incubated at 37°C for 1 h prior to transfer to TZM-bl reporter cells. Virus entry was measured by luciferase production 48 h after infection and normalized to luciferase expression in the absence of MAb. Non-V3-specific HIV-1 MAbs are represented by black lines. HIV-1 V3-specific MAbs (447-52D and F425 B4e8) are shown by red lines. IC50 neutralization values are presented in Table 1. Data represented are the mean and standard deviation of three independent experiments.